Author:
Filtenborg O,Frisvad J C,Svendsen J A
Abstract
A simple screening method for molds producing the intracellular mycotoxins brevianamide A, citreoviridin, cyclopiazonic acid, luteoskyrin, penitrem A, roquefortine C, sterigmatocystin, verruculogen, viomellein, and xanthomegnin was developed. After removing an agar plug from the mold culture, the mycelium on the plug is wetted with a drop of methanol-chloroform (1:2). By this treatment the intracellular mycotoxins are extracted within seconds and transferred directly to a thin-layer chromatography plate by immediately placing the plug on the plate while the mycelium is still wet. After removal of the plug, known thin-layer chromatographic procedures are carried out. The substrate (Czapek yeast autolysate agar) and growth conditions (25 degrees C for 7 days) used by Penicillium taxonomists proved suitable for the production of the mycotoxins investigated when 60 known toxigenic isolates and 865 cultures isolated from foods and feedstuffs were tested with this screening method.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference41 articles.
1. Improved thin layer chromatographic method for the isolation and estimation of sterigmatocystin in grains;Athnasios A. K.;J. Assoc. Off. Anal. Chem.,1977
2. A multiple inoculation technique for the screening of fungal isolates for the evaluation of growth and mycotoxin production on agar substrates;Barr J. G.;J. Sci. Food Agric.,1975
3. Disposition of mycophenolic acid, brevianamide A, asperphenamate, and ergosterol in solid cultures of Penicillium brevicompactum;Bird B. A.;Appl. Environ. Microbiol.,1982
4. Aspergillus flavus Kontamination von Nussen, Mandeln und Mais mit bekannten Aflatoxin-Gehalten;Blaser P.;Lebensm. Wiss. Technol.,1981
5. A screening medium and method to detect several mycotoxins in mold cultures;Bullerman L. B.;J. Milk Food Technol.,1974
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