Stress by Heat Shock Induces Massive Down Regulation of Genes and Allows Differential Allelic Expression of the Gal/GalNAc Lectin in Entamoeba histolytica

Author:

Weber Christian1,Guigon Ghislaine2,Bouchier Christiane2,Frangeul Lionel2,Moreira Sandrine2,Sismeiro Odile2,Gouyette Catherine2,Mirelman David3,Coppee Jean Yves2,Guillén Nancy1

Affiliation:

1. Unité Biologie Cellulaire du Parasitisme, INSERM 786, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France

2. Pasteur Genopole Île-de-France, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France

3. Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, Israel

Abstract

ABSTRACT Gene expression analysis by microarray assay revealed that when exposed to stress, Entamoeba histolytica exhibits a specific heat shock response, together with a dramatic overall reduction in gene transcription as well as differential allelic expression of key genes participating in virulence, such as the galactose/ N -acetylgalactosamine (Gal/GalNAc) lectin.

Publisher

American Society for Microbiology

Subject

Molecular Biology,General Medicine,Microbiology

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