Affiliation:
1. Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York 14642
Abstract
ABSTRACT
Recently, M. Dmitrova et al. (Mol. Gen. Genet. 257:205–212, 1998) described a LexA-based genetic system to monitor protein-protein interactions in an
Escherichia coli
background. However, the plasmids used in this system, pMS604 and pDP804, were not readily amenable for general use. In this report, we describe modifications of both plasmids that allow fragments of DNA to be fused to either vector in any reading frame. Homodimerization and heterodimerization of full-length proteins involved in polysialic acid synthesis in
E. coli
K1, as well as heterodimerization between a full-length protein and a protein fragment, demonstrate the usefulness of the modified plasmids for investigating bacterial protein-protein interactions in vivo.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
58 articles.
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