Affiliation:
1. Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan
Abstract
Stutzenberger, Fred
J. (Michigan State University, East Lansing),
Charles L. San Clemente, and Dharam V. Vadehra
. Nephelometric assay of staphylococcal coagulase. J. Bacteriol.
92:
1005–1009. 1966.—Clotting of fibrinogen by staphylococcal coagulase was accompanied by an increase in light scattering; this property was used as a basis for a new nephelometric method. Reaction rates, which were now easily and precisely measured, were found to be directly proportional to coagulase concentration, when optimal conditions were maintained. These conditions included
p
H and concentrations of fibrinogen, coagulase-reacting factor, and sodium chloride in the reaction mixture. A standardized procedure for the assay is outlined, and a unit for the expression of activity is proposed.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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