Standardized Procedure for Determination of Staphylocoagulase-Reference-Cited by-同舟云学术

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Standardized Procedure for Determination of Staphylocoagulase

Published:1969-04 Issue:4 Volume:17 Page:556-558
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Wegrzynowicz Z.¹,Jeljaszewicz J.¹,Lipinski B.¹

Affiliation:

1. State Institute of Hygiene, Department of Bacteriology, Warsaw 36, Poland

Abstract

A simple standardized procedure for the determination of staphylocoagulase, based on thrombin clotting time measurement with euglobulin, has been developed. A standard euglobulin solution containing 0.25% of clottable protein, 0.01 M ε-aminocaproic acid, and 20 units of heparin per ml is employed. One unit of staphylocoagulase is defined as the amount of the enzyme which clots this standard euglobulin solution in 25 sec.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.17.4.556-558.1969

Reference7 articles.

1. Staphylococcal coagulases;Jeljaszewicz J.;Postepy Hig. Med. Dogwiadczalnej,1958

2. Studies on staphylococcal coagulases. I. Methods for the determination of free and bound coagulases;Jeljaszewicz J.;Acta Microbiol. Polon.,1958

3. Studies on staphylococcal coagulases. II. Purification of free coagulase;Jeljaszewicz J.;Med. Doswiadczalna Mikrobiol.,1958

4. The purification of human fibrinogen;Kekwick R. A.;Biochem. J.,1955

5. Quick A. J. 1951. The physiology and pathology of hemostasis. Kimpton London.

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