Direct Production of Ethanol from Raw Corn Starch via Fermentation by Use of a Novel Surface-Engineered Yeast Strain Codisplaying Glucoamylase and α-Amylase-Reference-Cited by-同舟云学术

Direct Production of Ethanol from Raw Corn Starch via Fermentation by Use of a Novel Surface-Engineered Yeast Strain Codisplaying Glucoamylase and α-Amylase

Published:2004-08 Issue:8 Volume:70 Page:5037-5040
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Shigechi Hisayori¹,Koh Jun¹,Fujita Yasuya²,Matsumoto Takeshi²,Bito Yohei¹,Ueda Mitsuyoshi³,Satoh Eiichi⁴,Fukuda Hideki²,Kondo Akihiko¹

Affiliation:

1. Department of Chemical Science and Engineering, Faculty of Engineering

2. Division of Molecular Science, Graduate School of Science and Technology, Kobe University, Nada-ku, Kobe 657-8501

3. Department of Applied Biochemistry, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502

4. Department of Applied Biology and Chemistry, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan

Abstract

ABSTRACT Direct and efficient production of ethanol by fermentation from raw corn starch was achieved by using the yeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis α-amylase by using the C-terminal-half region of α-agglutinin and the flocculation functional domain of Flo1p as the respective anchor proteins. In 72-h fermentation, this strain produced 61.8 g of ethanol/liter, with 86.5% of theoretical yield from raw corn starch.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.70.8.5037-5040.2004

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