A Simple Method To Detect Point Mutations in Aspergillus fumigatus cyp51A Gene Using a Surveyor Nuclease Assay-Reference-Cited by-同舟云学术

A Simple Method To Detect Point Mutations in Aspergillus fumigatus cyp51A Gene Using a Surveyor Nuclease Assay

Published:2020-03-24 Issue:4 Volume:64 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Arai Teppei¹,Majima Hidetaka¹²,Watanabe Akira¹,Kamei Katsuhiko¹

Affiliation:

1. Medical Mycology Research Center, Chiba University, Chiba, Japan

2. Department of Respiratory Medicine, Tokyo Medical and Dental University, Tokyo, Japan

Abstract

One of the main mechanisms of azole resistance of Aspergillus fumigatus is thought to be a reduction in the drug’s affinity for the target molecule, Cyp51A, due to its amino acid mutation(s). It is known that the azole resistance pattern is closely related to the mutation site(s) of the molecule. In this study, we tried to develop a simple and rapid detection method for cyp51A mutations using the endonuclease Surveyor nuclease. The Surveyor nuclease assay was verified using several azole-resistant strains of A. fumigatus that possess point mutations in Cyp51A.

Funder

Japan Agency for Medical Research and Development

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.02271-19

Reference27 articles.

1. Azole-Resistant Aspergillosis: Epidemiology, Molecular Mechanisms, and Treatment

2. Epidemiological and Genomic Landscape of Azole Resistance Mechanisms in Aspergillus Fungi

3. In VitroBiochemical Study of CYP51-Mediated Azole Resistance in Aspergillus fumigatus