Multicenter Comparison of the Etest and EUCAST Methods for Antifungal Susceptibility Testing of Candida Isolates to Micafungin-Reference-Cited by-同舟云学术

Multicenter Comparison of the Etest and EUCAST Methods for Antifungal Susceptibility Testing of Candida Isolates to Micafungin

Published:2016-08 Issue:8 Volume:60 Page:5088-5091
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Bougnoux M.-E.¹,Dannaoui E.²^ORCID,Accoceberry I.³,Angoulvant A.⁴,Bailly E.⁵,Botterel F.⁶,Chevrier S.⁷,Chouaki T.⁸,Cornet M.⁹^ORCID,Dalle F.¹⁰,Datry A.¹¹,Dupuis A.¹,Fekkar A.¹¹¹²,Gangneux J. P.⁷,Guitard J.¹²¹³,Hennequin C.¹²¹³,Le Govic Y.¹⁴,Le Pape P.¹⁵,Maubon D.⁹,Ranque S.¹⁶^ORCID,Sautour M.¹¹,Sendid B.¹⁷,Chandenier J.⁵¹⁸

Affiliation:

1. Université Paris-Descartes, Faculté de Médecine, and Unité de Parasitologie-Mycologie, Service de Microbiologie, Hôpital Necker Enfants Malades, Assistance Publique—Hôpitaux de Paris, Paris, France

2. Université Paris-Descartes, Faculté de Médecine, and Unité de Parasitologie-Mycologie, Service de Microbiologie, Hôpital Européen Georges Pompidou, Assistance Publique—Hôpitaux de Paris, Paris, France

3. Laboratoire de Parasitologie-Mycologie, Centre hospitalier Universitaire de Bordeaux, Bordeaux, France

4. Assistance Publique—Hôpitaux de Paris, Hôpital de Bicêtre, Laboratoire de Parasitologie-Mycologie, and Université Paris-Sud, Faculté de Médecine, Le Kremlin-Bicêtre, CNRS UMR 8621, Génétique Quantitative et Evolution, Le Moulon, France

5. Service de Parasitologie-Mycologie-Médecine tropicale Centre Hospitalier Régional Universitaire, Tours, France

6. Hôpital Henri Mondor, DHU VIC, APHP, and EA 7380, DYNAMYC, Université Paris-Est Créteil, Créteil, France

7. Service de Parasitologie-Mycologie, Centre Hospitalier Universitaire, and Université Rennes 1 Inserm U1085—IRSET, Rennes, France

8. Centre Hospitalo-Universitaire, Amiens, France

9. Laboratoire de Parasitologie-Mycologie, Centre Hospitalo-Universitaire Grenoble, and Université Grenoble Alpes, Grenoble, France

10. Laboratoire de Parasitologie Mycologie, Centre Hospitalo-Universitaire, and Université Bourgogne—Franche Comté, UMR PAM, Dijon, France

11. AP-HP, Groupe Hospitalier La Pitié-Salpêtrière, Service de Parasitologie Mycologie, Paris, France

12. Sorbonne Universités, UPMC Univ Paris 06, INSERM U1135, CNRS ERL 8255, Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris), Paris, France

13. AP-HP, Hôpital St Antoine, Service de Parasitologie-Mycologie, Paris, France

14. Laboratoire de Parasitologie-Mycologie, Institut de Biologie en Santé-PBH, Centre Hospitalier Universitaire, Angers, France

15. Laboratoire de Parasitologie-Mycologie, Institut de Biologie, Centre Hospitalier Universitaire de Nantes, Nantes, France

16. Parasitologie & Mycologie, Hôpital de la Timone, Assistance Publique-Hôpitaux de Marseille, and IP-TPT UMR MD3, Aix-Marseille Université, Marseille, France

17. Centre Hospitalier Universitaire de Lille, Université de Lille, Inserm U995-Team 2, and Inflammation Research International Center (LIRIC), Lille, France

18. Université François Rabelais, INSERM U1100, Centre d'Étude des Pathologies Respiratoires (CEPR), Tours, France

Abstract

ABSTRACT In vitro susceptibility of 933 Candida isolates, from 16 French hospitals, to micafungin was determined using the Etest in each center. All isolates were then sent to a single center for determination of MICs by the EUCAST reference method. Overall essential agreement between the two tests was 98.5% at ±2 log 2 dilutions and 90.2% at ±1 log 2 dilutions. Categorical agreement was 98.2%. The Etest is a valuable alternative to EUCAST for the routine determination of micafungin MICs in medical mycology laboratories.

Funder

Astellas Pharma

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00630-16

Reference22 articles.

1. Comparative In Vitro Activities of Caspofungin and Micafungin, Determined Using the Method of the European Committee on Antimicrobial Susceptibility Testing, against Yeast Isolates Obtained in France in 2005-2006

2. Susceptibility to echinocandins of Candida spp. strains isolated in Italy assessed by European Committee for Antimicrobial Susceptibility Testing and Clinical Laboratory Standards Institute broth microdilution methods

3. Multicenter Study of Anidulafungin and Micafungin MIC Distributions and Epidemiological Cutoff Values for Eight Candida Species and the CLSI M27-A3 Broth Microdilution Method