Affiliation:
1. Department of Applied Microbiology, Nippon Roche Research Center, Kamakura 247, Japan
Abstract
ABSTRACT
The NADPH-dependent
l
-sorbose reductase (SR) of
l
-sorbose-producing
Gluconobacter suboxydans
IFO 3291 contributes to intracellular
l
-sorbose assimilation. The gene disruptant showed no SR activity and did not assimilate the once-produced
l
-sorbose, indicating that the SR functions mainly as an
l
-sorbose-reducing enzyme in vivo and not as a
d
-sorbitol-oxidizing enzyme.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference12 articles.
1. Adachi, O., Y. Ano, D. Moonmangmee, E. Shinagawa, H. Toyama, G. Theeragool, N. Lotong, and K. Matsushita. 1999. Crystallization and properties of NADPH-dependent l-sorbose reductase from Gluconobacter melanogenus IFO 3294. Biosci. Biotechnol. Biochem.63:2137–2143.
2. Hoshino, T., T. Sugisawa, M. Tazoe, M. Shinjoh, and A. Fujiwara. 1990. Metabolic pathway for 2-keto-l-gulonic acid formation in Gluconobacter melanogenus IFO 3293. Agric. Biol. Chem.54:1211–1218.
3. Manning R. F. and M. Kahn. January 1992. Biosynthesis of 2-keto- l -gulonic acid. U.S. patent 5 082 785.
4. Reichstein, T., A. Grüssner, and R. Oppenauer. 1933. Synthese der d- und l-ascorbinsäure (C-Vatamin). Helv. Chem. Acta16:1019–1033.
5. Schneider, K. H., F. Giffhorn, and S. Kaplan. 1993. Cloning, nucleotide sequence and characterization of the mannitol dehydrogenase gene from Rhodobacter sphaeroides. J. Gen. Microbiol.139:2475–2484.
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