Virulence of Vancomycin-Resistant Enterococcus faecium According to Linezolid Resistance and Clinical Outbreak Status

Abstract

ABSTRACTAssessing clinical virulence differences between vancomycin-resistantEnterococcus faecium(VREF) strains resistant to linezolid (LRVRE) and linezolid-susceptible VRE (LSVRE) strains is difficult due to confounding patient variables.Galleria mellonellais a validated host interaction model allowing straightforward organism virulence assessment. The objective of this study was to assess the virulence of VREF inG. mellonellaaccording to linezolid resistance and clinical outbreak status. A genetically related pair of VREF strains with and without genotypically confirmed linezolid resistance was selected for analysis. Additionally, six strains of LSVRE and two strains of LRVRE were selected according to epidemiologic outbreak status. Mortality ofG. mellonellawas assessed daily over a 5-day period and analyzed using Kaplan-Meier survival curves and log rank tests. Linezolid resistance did not have a significant effect onG. mellonellamortality in the genetically related pair (P= 0.93). There was no significant difference in mortality over time between strains (non-outbreak [i.e., no patient transmissions were recorded] [n= 2] versus outbreak [i.e., transmission occurred between 3 or more patients in a period of 30 days] [n= 6],P= 0.84; extensive transmission [i.e., the isolate was transmitted between at least 80 patients] [n= 2] versus limited transmission [i.e., the isolate was transmitted between fewer than 10 patients] [n= 4],P= 0.78). These results suggest that patients infected with LRVRE or outbreak strains of VREF are at no greater risk of poor outcomes mediated by organism virulence than those infected with LSVRE or non-outbreak strains.