Affiliation:
1. Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium
2. Tuberculosis Laboratory, Instituto Nacional de Laboratorios de Salud, Miraflores, La Paz, Bolivia
Abstract
ABSTRACT
The emergence of multidrug-resistant tuberculosis calls for new, rapid drug susceptibility tests. We have tested 150
Mycobacterium tuberculosis
isolates against the second-line drugs ethionamide, kanamycin, capreomycin, ofloxacin, and
para
-aminosalicylic acid by the colorimetric resazurin microtiter assay and the proportion method. By visual reading, MICs were obtained after 8 days. A very good correlation between results by the colorimetric resazurin microtiter assay and the proportion method was obtained. The colorimetric resazurin microtiter assay is inexpensive, rapid, and simple to perform, and implementation of the assay is feasible for low-resource countries.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
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