Kanamycin Susceptibility Testing of Mycobacterium tuberculosis Using Mycobacterium Growth Indicator Tube and a Colorimetric Method-Reference-Cited by-同舟云学术

Kanamycin Susceptibility Testing of Mycobacterium tuberculosis Using Mycobacterium Growth Indicator Tube and a Colorimetric Method

Published:2001-06 Issue:6 Volume:45 Page:1934-1936
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Bastian Ivan¹²,Rigouts Leen¹,Palomino Juan Carlos¹,Portaels Françoise¹

Affiliation:

1. Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium,1 and

2. Institute of Medical & Veterinary Science, Adelaide, Australia2

Abstract

ABSTRACT Two novel systems were evaluated for performing indirect kanamycin susceptibility tests on 72 strains of Mycobacterium tuberculosis . The microplate Alamar blue colorimetric method (breakpoint, 2.5 μg/ml) and the Mycobacterium Growth Indicator Tube (MGIT) system (breakpoint, 5.0 μg/ml) both produced 98.6% agreement when compared with the conventional proportion method performed on 7H10 agar using 5.0 μg of kanamycin/ml. Both systems provided results within an average of 1 week.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.45.6.1934-1936.2001

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