Evaluation of Dried Whole Blood Spots Obtained by Heel or Finger Stick as an Alternative to Venous Blood for Diagnosis of Human Immunodeficiency Virus Type 1 Infection in Vertically Exposed Infants in the Routine Diagnostic Laboratory-Reference-Cited by-同舟云学术

Evaluation of Dried Whole Blood Spots Obtained by Heel or Finger Stick as an Alternative to Venous Blood for Diagnosis of Human Immunodeficiency Virus Type 1 Infection in Vertically Exposed Infants in the Routine Diagnostic Laboratory

Published:2007-02 Issue:2 Volume:14 Page:201-203
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Patton Janet C.¹,Akkers Eveline²³,Coovadia Ashraf H.¹⁴,Meyers Tammy M.¹⁴,Stevens Wendy S.²³,Sherman Gayle G.¹²³

Affiliation:

1. Wits Pediatric HIV Clinics, Wits Health Consortium

2. Departments of Molecular Medicine and Hematology

3. National Health Laboratory Service, Johannesburg, Gauteng, South Africa

4. Pediatrics, University of the Witwatersrand, Johannesburg, Gauteng, South Africa

Abstract

ABSTRACT The diagnostic accuracy of the Roche Amplicor human immunodeficiency virus type 1 DNA PCR assay (version 1.5) on DNA extracted from pediatric heel prick dried blood spots using Roche MagNA Pure nucleic acid purification technology was evaluated. The methodologies transfer successfully from the labor-intensive research laboratory to the high-throughput automated routine laboratory.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CVI.00223-06

Reference18 articles.

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2. Stability of dried blood spot specimens for detection of human immunodeficiency virus DNA by polymerase chain reaction

3. Rapid screening for early detection of mother-to-child transmission of human immunodeficiency virus type 1

4. Cassol, S., W. G. Weniger, P. G. Babu, M. O. Salminen, X. Zheng, M. T. Htoon, A. Delaney, M. O'Shaughnessy, and C. Y. Ou. 1996. Detection of HIV type 1 env subtypes A, B, C and E in Asia using dried blood spots: a new surveillance tool for molecular epidemiology. AIDS Res. Hum. Retrovir.12:1435-1441.

5. Centers for Disease Control and Prevention. 1994. Revised classification system for human immunodeficiency virus infection in children less than 13 years of age. Morb. Mortal. Wkly. Rep.43:1-10.

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