Development and Validation of a Multiplex Microsphere Immunoassay Using Dried Blood Spots for SARS-CoV-2 Seroprevalence: Application in First Responders in Colorado, USA-Reference-Cited by-同舟云学术

Development and Validation of a Multiplex Microsphere Immunoassay Using Dried Blood Spots for SARS-CoV-2 Seroprevalence: Application in First Responders in Colorado, USA

Published:2021-05-19 Issue:6 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Schultz Jonathan S.¹^ORCID,McCarthy Mary K.²,Rester Cody²,Sabourin Katherine R.²,Annen Kyle³,DomBourian Melkon³,Eisenmesser Elan⁴,Frazer-Abel Ashley⁵,Knight Vijaya³,Jaenisch Thomas⁶,Morrison Thomas E.²,Rochford Rosemary²⁶,Kedl Ross M.²

Affiliation:

1. Division of Infectious Diseases, School of Medicine, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USA

2. Department of Immunology and Microbiology, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USA

3. Clinical & Translational Allergy and Immunology Laboratory, Children’s Hospital of Colorado, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USA

4. Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USA

5. Exsera Biolabs, Department of Rheumatology, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USA

6. Department of Epidemiology, Colorado School of Public Health, Anschutz Medical Campus, Aurora, Colorado, USA

Abstract

Serological testing of large representative populations for antibodies to SARS-CoV-2 is needed to estimate seroprevalence, transmission dynamics, and the duration of antibody responses from natural infection and vaccination. In this study, a high-throughput SARS-CoV-2 multiplex microsphere immunoassay (MMIA) was developed for the receptor binding domain (RBD) and nucleocapsid (N) that was more sensitive than enzyme-linked immunosorbent assay (ELISA) (98% versus 87%).

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00290-21

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