Affiliation:
1. Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115
Abstract
ABSTRACT
Quorum sensing (QS) has been previously shown to play an important role in the development of
Pseudomonas aeruginosa
biofilms (D. G. Davies et al., Science 280:295-298, 1998). Although QS regulation of swarming and DNA release has been shown to play important roles in biofilm development, regulation of genes directly involved in biosynthesis of biofilm matrix has not been described. Here, transcription of the
pel
operon, essential for the production of a glucose-rich matrix exopolysaccharide, is shown to be greatly reduced in
lasI
and
rhlI
mutants. Chemical complementation of the
lasI
mutant with 3-oxo-dodecanoyl homoserine lactone restores
pel
transcription to the wild-type level and biofilm formation ability. These findings thus connect QS signaling and transcription of genes responsible for biofilm matrix biosynthesis.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
285 articles.
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