Affiliation:
1. Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15
2. Centre d'Etudes Pharmaceutiques, ChÂtenay-Malabry, France
Abstract
ABSTRACT
A self-transferable plasmid of ca. 80 kb, pIP1204, conferred multiple-antibiotic resistance to
Klebsiella pneumoniae
BM4536, which was isolated from a urinary tract infection. Resistance to β-lactams was due to the
bla
TEM1
and
bla
CTX-M
genes, resistance to trimethroprim was due to the
dhfrXII
gene, resistance to sulfonamides was due to the
sul1
gene, resistance to streptomycin-spectinomycin was due to the
ant3"9
gene, and resistance to nearly all remaining aminoglycosides was due to the
aac3-II
gene and a new gene designated
armA
(aminoglycoside resistance methylase). The cloning of
armA
into a plasmid in
Escherichia coli
conferred to the new host high-level resistance to 4,6-disubstituted deoxystreptamines and fortimicin. The deduced sequence of ArmA displayed from 37 to 47% similarity to those of 16S rRNA m
7
G methyltransferases from various actinomycetes, which confer resistance to aminoglycoside-producing strains. However, the low guanine-plus-cytosine content of
armA
(30%) does not favor an actinomycete origin for the gene. It therefore appears that posttranscriptional modification of 16S rRNA can confer high-level broad-range resistance to aminoglycosides in gram-negative human pathogens.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
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