Affiliation:
1. Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, 3001 Leuven, Belgium
Abstract
ABSTRACT
Lactobacillus rhamnosus
GG (ATCC 53103) is one of the clinically best-studied probiotic organisms. Moreover,
L. rhamnosus
GG displays very good in vitro adherence to epithelial cells and mucus. Here, we report that
L. rhamnosus
GG is able to form biofilms on abiotic surfaces, in contrast to other strains of the
Lactobacillus casei
group tested under the same conditions. Microtiter plate biofilm assays indicated that in vitro biofilm formation by
L. rhamnosus
GG is strongly modulated by culture medium factors and conditions related to the gastrointestinal environment, including low pH; high osmolarity; and the presence of bile, mucins, and nondigestible polysaccharides. Additionally, phenotypic analysis of mutants affected in exopolysaccharides (
wzb
), lipoteichoic acid (
dltD
), and central metabolism (
luxS
) showed their relative importance in biofilm formation by
L. rhamnosus
GG.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
218 articles.
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