Dam Methyltransferase Is Required for Stable Lysogeny of the Shiga Toxin (Stx2)-Encoding Bacteriophage 933W of Enterohemorrhagic Escherichia coli O157:H7-Reference-Cited by-同舟云学术

Dam Methyltransferase Is Required for Stable Lysogeny of the Shiga Toxin (Stx2)-Encoding Bacteriophage 933W of Enterohemorrhagic Escherichia coli O157:H7

Published:2008-01 Issue:1 Volume:190 Page:438-441
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Murphy Kenan C.¹,Ritchie Jennifer M.²,Waldor Matthew K.²,Løbner-Olesen Anders³,Marinus M. G.⁴

Affiliation:

1. Molecular Genetics and Microbiology

2. The Channing Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115

3. Department of Science, Systems and Models, Roskilde University, DK-4000 Roskilde, Denmark

4. Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605

Abstract

ABSTRACT Shiga toxin 2 (Stx2), one of the principal virulence factors of enterohemorrhagic Escherichia coli , is encoded by 933W, a lambda-like prophage. 933W prophage induction contributes to Stx2 production, and here, we provide evidence that Dam methyltransferase is essential for maintenance of 933W lysogeny. Our findings are consistent with the idea that the 933W prophage has a relatively low threshold for induction, which may promote Stx2 production during infection.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.01373-07

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