The C-Terminal Domain of Cernunnos/XLF Is Dispensable for DNA Repair In Vivo-Reference-Cited by-同舟云学术

The C-Terminal Domain of Cernunnos/XLF Is Dispensable for DNA Repair In Vivo

Published:2009-03 Issue:5 Volume:29 Page:1116-1122
ISSN:0270-7306
Container-title:Molecular and Cellular Biology
language:en
Short-container-title:Mol Cell Biol

Author:

Malivert Laurent¹²,Callebaut Isabelle³,Rivera-Munoz Paola¹²,Fischer Alain¹⁴⁵,Mornon Jean-Paul³,Revy Patrick¹⁵,de Villartay Jean-Pierre¹⁴⁵

Affiliation:

1. INSERM, Hôpital Necker-Enfants Malades, U768, Unité de Développement Normal et Pathologique du Système Immunitaire

2. Université Paris Diderot—Paris 7, Bâtiment les Grands Moulins, 16 rue Marguerite Duras, 75205 Paris Cedex 13

3. Département de Biologie Structurale, IMPMC, CNRS UMR7590, Universités Paris 6 et 7

4. Assistance Publique-Hôpitaux de Paris, Hôpital Necker-Enfants Malades, Unité d'Immunologie et d'Hématologie, F-75015 Paris

5. Université Paris-Descartes, Faculté de Médecine René Descartes, F-75005 Paris, France

Abstract

ABSTRACT The core nonhomologous end-joining DNA repair pathway is composed of seven factors: Ku70, Ku80, DNA-PKcs, Artemis, XRCC4 (X4), DNA ligase IV (L4), and Cernunnos/XLF (Cernunnos). Although Cernunnos and X4 are structurally related and participate in the same complex together with L4, they have distinct functions during DNA repair. L4 relies on X4 but not on Cernunnos for its stability, and L4 is required for optimal interaction of Cernunnos with X4. We demonstrate here, using in vitro-generated Cernunnos mutants and a series of functional assays in vivo, that the C-terminal region of Cernunnos is dispensable for its activity during DNA repair.

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/MCB.01521-08

Reference23 articles.

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3. Andres, S. N., M. Modesti, C. J. Tsai, G. Chu, and M. S. Junop. 2007. Crystal structure of human XLF: a twist in nonhomologous DNA end-joining. Mol. Cell28:1093-1101.

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