Duplication of a Truncated Paralog of the Family B DNA Polymerase Gene Aa-polB in the Agrocybe aegerita Mitochondrial Genome

Author:

Barroso Gerard1,Bois Frederic1,Labarère Jacques1

Affiliation:

1. Laboratoire de Génétique Moléculaire et d'Amélioration des Champignons Cultivés, Université Victor Segalen Bordeaux 2—INRA, I.B.V.M., CRA de Bordeaux, 33883 Villenave d'Ornon Cédex, France

Abstract

ABSTRACT The Agrocybe aegerita mitochondrial genome contains a truncated family B DNA polymerase gene ( Aa-polB P1 ) whose nucleotide sequence is 86% identical to the previously described and potentially functional Aa-polB gene. A tRNA Met gene occurs at the 3′ end of the Aa-polB P1 gene. The Aa-polB P1 gene could result from reverse transcription of an Aa-polB mRNA primed by a tRNA Met followed by the integration of the cDNA after recombination at the mitochondrial tRNA locus. Two naturally occurring alleles of Aa-polB P1 carry one or two copies of the disrupted sequence. In strains with two copies of Aa-polB P1 , these copies are inverted relative to one another and separated by a short sequence carrying the tRNA Met gene. Both A. aegerita mitochondrial family B DNA polymerases were found to be related to other family B DNA polymerases (36 to 53% amino acid similarity), including the three enzymes of the archaebacterium Sulfolobus solfataricus . If mitochondria originated from a fusion between a Clostridium -like eubacterium and a Sulfolobus -like archaebacterium, then the A. aegerita family B DNA polymerase genes could be remnants of the archaebacterial genes.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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