Author:
Hageman J H,Shankweiler G W,Wall P R,Franich K,McCowan G W,Cauble S M,Grajeda J,Quinones C
Abstract
The composition and application of a single, chemically defined medium or growth and sporulation of Bacillus subtilis is described. At 37 degrees C cells grew with a doubling time of about 40 min; cultures attained near-maximal spore formation (70 to 80% by 12 h after the end of exponential growth and produced 1 X 10(9) to 2 X 10(9) heat-resistant free spores at 24 h. Dipicolinic acid production was completed between 7 and 11 h. Cells grown in the single, chemically defined medium excreted levels of serine and neutral proteases comparable to those excreted in nutrient broth medium.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
79 articles.
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