Affiliation:
1. Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo, North Dakota 58105
2. Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa 50011
Abstract
ABSTRACT
Nontyphoidal salmonellae are among the leading causes of food-borne disease in the United States. Because of the importance of
Salmonella enterica
in food-borne disease, numerous typing methodologies have been developed. Among the several molecular typing methods, pulsed-field gel electrophoresis (PFGE) is currently considered the “gold standard” technique in typing
Salmonella
. The aim of this study was to compare the discriminatory power of PFGE to multilocus sequence typing (MLST) in typing
Salmonella enterica
serovar Typhimurium clinical isolates. A total of 85
Salmonella
Typhimurium clinical isolates from cattle were used in this study. PFGE using XbaI was performed on the 85 isolates by the Centers for Disease Control and Prevention method, and data were analyzed using the BioNumerics software package. Fifty PFGE profiles were observed among the isolates, and these grouped into three major clusters. For the MLST analysis, the
manB
,
pduF
,
glnA
, and
spaM
genes were amplified by PCR from the same 85 isolates. DNA sequencing of these four genes,
manB
,
pduF
,
glnA
, and
spaM
, showed no genetic diversity among the isolates tested, with a 100% identity in nucleotide sequence. Moreover, the DNA sequences of the aforementioned genes showed 100% identity to the sequence reported in GenBank for the
S. enterica
serovar Typhimurium LT2 strain. Therefore, MLST, using these genes, lacks the discriminatory power of PFGE for typing
Salmonella enterica
serovar Typhimurium.
Publisher
American Society for Microbiology
Cited by
79 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献