Affiliation:
1. Departments
of Zoology
2. Department of Biological
Sciences, Lancaster University, Lancaster LA1 4YQ,United Kingdom
3. Biological
Anthropology, University of Oxford, Oxford
OX1 3SY
Abstract
ABSTRACT
The
genetic diversity of
Campylobacter jejuni
isolates from farm
animals and their environment was investigated by multilocus sequence
typing (MLST). A total of 30 genotypes, defined by allelic profiles
(assigned to sequence types [STs]), were found in 112
C.
jejuni
isolates originating in poultry, cattle, sheep, starlings,
and slurry. All but two of these genotypes belonged to one of nine
C. jejuni
clonal complexes previously identified in isolates
from human disease and retail food samples and one clonal complex
previously associated with an environmental source. There was some
evidence for the association of certain clonal complexes with
particular farm animals: isolates belonging to the ST-45 complex
predominated among poultry isolates but were absent among sheep
isolates, while isolates belonging to the ST-61 and ST-42 complexes
were predominant among sheep isolates but were absent from the poultry
isolates. In contrast, ST-21 complex isolates were distributed among
the different isolation sources. Comparison with MLST data from 91
human disease isolates showed small but significant genetic
differentiation between the farm and human isolates; however,
representatives of six clonal complexes were found in both samples.
These data demonstrate that MLST and the clonal complex model can be
used to identify and compare the genotypes of
C. jejuni
isolates from farm animals and the environment with those from retail
food and human
disease.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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