Amikacin Assay in Serum by High-Performance Liquid Chromatography-Reference-Cited by-同舟云学术

Amikacin Assay in Serum by High-Performance Liquid Chromatography

Published:1978-12 Issue:6 Volume:14 Page:880-885
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Maitra Shyamal K.¹²,Yoshikawa Thomas T.³²,Steyn Catherine M.¹²,Guze Lucien B.¹³²,Schotz Michael C.¹²

Affiliation:

1. Research Service, Veterans Administration, Wadsworth Hospital Center, Los Angeles, California 90073

2. UCLA School of Medicine, Los Angeles, California 90024

3. Departments of Medicine, Harbor General Hospital, Torrance, California 90509

Abstract

A high-performance liquid chromatographic method for the quantitative determination of amikacin in serum is described. The antibiotic was separated from serum by adsorption on silica gel. The adsorbed drug was then derivatized with o -phthalaldehyde and eluted with ethanol. The derivatized amikacin was separated by reverse-phase, high-performance liquid chromatography and quantitated by fluorometry. A linear response for serum samples containing amikacin ranging from 1.0 to 15 μg/ml was obtained. Other antibiotics including various aminoglycosides did not interfere with the amikacin assay. Comparison with a standard microbiological assay gave a correlation coefficient of 0.99. This chemical assay is sensitive and specific under the conditions tested and can be performed rapidly.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.14.6.880

Reference13 articles.

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