Affiliation:
1. Department of Laboratory Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55901
Abstract
A procedure is described that uses high-pressure liquid chromatography for assay of gentamicin in serum. The technique involves extraction of gentamicin from serum by using a CM-Sephadex column and analysis by reverse-phase, ion-pair chromatography. Continuous-flow, post-column derivatization with
o
-phthaladehyde is used to form fluorescent products for detection. The possible scope of this method for analysis of other aminoglycoside antibiotics is illustrated by application to sisomicin and netilmicin.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Reference10 articles.
1. o-Phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin;Benson J. R.;Proc. Natl. Acad. Sci. U.S.A.,1975
2. Assay of flucytosine and furosemide by high-pressure liquid chromatography;Blair A. D.;J. Pharm. Sci.,1975
3. Ion-pair extraction and high-speed liquid chromatography of cephalothin and deacetylcephalothin in human serum and urine;Cooper M. J.;Drug Metab. Dispos.,1973
4. Hine J. 1962. Physical organic chemistry p. 73-75. McGraw-Hill New York.
5. High-performance liquid chromatographic determination of kanamycin;Mays D. L.;J. Chromatogr.,1976
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