In Vitro Susceptibilities of Madurella mycetomatis to Itraconazole and Amphotericin B Assessed by a Modified NCCLS Method and a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5- Sulfophenyl)-5-[(Phenylamino)Carbonyl]-2 H - Tetrazolium Hydroxide (XTT) Assay-Reference-Cited by-同舟云学术

In Vitro Susceptibilities of Madurella mycetomatis to Itraconazole and Amphotericin B Assessed by a Modified NCCLS Method and a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5- Sulfophenyl)-5-[(Phenylamino)Carbonyl]-2 H - Tetrazolium Hydroxide (XTT) Assay

Published:2004-07 Issue:7 Volume:48 Page:2742-2746
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Ahmed Abdalla O. A.¹,van de Sande Wendy W. J.²,van Vianen Wim²,van Belkum Alex²,Fahal Ahmed H.¹,Verbrugh Henri A.²,Bakker-Woudenberg Irma A. J. M.²

Affiliation:

1. Mycetoma Research Group, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan

2. Department of Medical Microbiology & Infectious Diseases, Erasmus MC University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands

Abstract

ABSTRACT Susceptibilities of Madurella mycetomatis against amphotericin B and itraconazole in vitro were determined by protocols based on NCCLS guidelines (visual reading) and a 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2 H -tetrazolium hydroxide (XTT) assay for fungal viability. The XTT assay was reproducible and sensitive for both antifungals. Itraconazole (MIC at which 50% of the isolates tested are inhibited [MIC 50 ]) of 0.06 to 0.13 mg/liter) was superior to amphotericin B (MIC 50 of 0.5 to 1.0 mg/liter).

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.48.7.2742-2746.2004

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