Molecular Detection and Identification of Agents of Eumycetoma: Detailed Report of Two Cases

Author:

Ahmed Abdalla O. A.12,Desplaces Nicole3,Leonard Philippe4,Goldstein Fred5,De Hoog Sybren6,Verbrugh Henri2,van Belkum Alex2

Affiliation:

1. Mycetoma Research Group, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan

2. Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam

3. Department of Microbiology

4. Department of Orthopedic Surgery, Croix Saint Simon Hospital

5. Department of Microbiology, Saint Joseph Hospital, Paris, France

6. Centraalbureau voor Schimmelcultures (CBS), Utrecht, The Netherlands

Abstract

ABSTRACT We describe two cases of eumycetoma in the legs. The infections could not be adequately diagnosed by classical mycology, but the causative agents were successfully identified as Madurella mycetomatis by species-specific PCR and DNA sequencing.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference13 articles.

1. Development of a Species-Specific PCR-Restriction Fragment Length Polymorphism Analysis Procedure for Identification of Madurella mycetomatis

2. Boiron, P., R. Locci, M. Goodfellow, S. A. Gumaa, K. Isik, B. Kim, M. M. McNeil, M. C. Salinas-Carmona, and H. Shojaei. 1998. Nocardia, nocardiosis and mycetoma. Med. Mycol.36:26-37.

3. Rapid and simple method for purification of nucleic acids

4. Borelli, D. 1957. Madurella mycetomi: fialides, fialospores. Inoculation al raton. Bol. Lab. Clin.2:1-8.

5. Borelli, D. 1962. Medios caseros para micologia. Arch. Venez. Med. Trop. Parasitol.4:301-310.

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