A Redox-Sensitive Thiol in Wis1 Modulates the Fission Yeast Mitogen-Activated Protein Kinase Response to H 2 O 2 and Is the Target of a Small Molecule-Reference-Cited by-同舟云学术

A Redox-Sensitive Thiol in Wis1 Modulates the Fission Yeast Mitogen-Activated Protein Kinase Response to H 2 O 2 and Is the Target of a Small Molecule

Published:2020-03-16 Issue:7 Volume:40 Page:
ISSN:0270-7306
Container-title:Molecular and Cellular Biology
language:en
Short-container-title:Mol Cell Biol

Author:

Sjölander Johanna J.¹,Tarczykowska Agata¹,Picazo Cecilia¹²,Cossio Itziar¹,Redwan Itedale Namro¹,Gao Chunxia¹,Solano Carlos¹,Toledano Michel B.³,Grøtli Morten¹,Molin Mikael¹²,Sunnerhagen Per¹^ORCID

Affiliation:

1. University of Gothenburg, Department of Chemistry and Molecular Biology, Gothenburg, Sweden

2. Chalmers University of Technology, Department of Biology and Biological Engineering, Gothenburg, Sweden

3. Oxidative Stress and Cancer Laboratory, Integrative Biology and Molecular Genetics Unit, CEA Saclay, Gif-sur-Yvette, France

Abstract

Oxidation of a highly conserved cysteine (Cys) residue located in the kinase activation loop of mitogen-activated protein kinase kinases (MAPKK) inactivates mammalian MKK6. This residue is conserved in the fission yeast Schizosaccharomyces pombe MAPKK Wis1, which belongs to the H 2 O 2 -responsive MAPK Sty1 pathway. Here, we show that H 2 O 2 reversibly inactivates Wis1 through this residue (C458) in vitro .

Funder

Swedish Research Council

Helge Axson Johnson's Foundation

Agence Nationale de la Recherche

Swedish Cancer Foundation

Lennander's Foundation

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/MCB.00346-19