Quantitative Immunocapture PCR Assay for Detection of Campylobacter jejuni in Foods

Author:

Waller David F.1,Ogata Steven A.1

Affiliation:

1. Hawaii Biotechnology Group, Inc., Aiea, Hawaii

Abstract

ABSTRACT The rapid detection of food-borne bacterial pathogens as part of a quality control program is necessary for the maintenance of a safe food supply. In this report, we present our findings for an immunocapture PCR method for the detection of Campylobacter jejuni in foods. The method permits direct detection of the pathogen without an enrichment step and can be performed in approximately 8 h. Assay results are quantitative, and one cell in a milliliter sample can be detected. Application of the method to spiked milk samples and chicken skin washes did not affect the sensitivity of the assay.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference22 articles.

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4. The magnetic immuno-polymerase chain reaction assay for the detection of Campylobacter in milk and poultry;Docherty L.;Lett. Appl. Microbiol.,1996

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