Radicicol-Mediated Inhibition of Topoisomerase VIB-VIA Activity of the Human Malaria Parasite Plasmodium falciparum-Reference-Cited by-同舟云学术

Radicicol-Mediated Inhibition of Topoisomerase VIB-VIA Activity of the Human Malaria Parasite Plasmodium falciparum

Published:2016-02-25 Issue:1 Volume:1 Page:
ISSN:2379-5042
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language:en
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Author:

Chalapareddy Sureshkumar¹,Chakrabarty Swati¹,Bhattacharyya Mrinal Kanti²,Bhattacharyya Sunanda¹

Affiliation:

1. Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, Hyderabad, India

2. Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India

Abstract

In this study we characterize topoisomerase VI from Plasmodium falciparum using genetic and biochemical approaches. We use various inhibitors and identify radicicol as a specific inhibitor of its decatenation activity. We establish a very simple and economical biochemical assay system that can be exploited to screen inhibitors of PfTopoVI.

Funder

Council of Scientific and Industrial Research

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mSphere.00025-15

Reference21 articles.

1. DNA Topoisomerase VI Is Essential for Endoreduplication in Arabidopsis

2. Genome sequence of the human malaria parasite Plasmodium falciparum

3. New Mechanistic and Functional Insights into DNA Topoisomerases