Determining the Genetic Structure of the Natural Population of Staphylococcus aureus : a Comparison of Multilocus Sequence Typing with Pulsed-Field Gel Electrophoresis, Randomly Amplified Polymorphic DNA Analysis, and Phage Typing-Reference-Cited by-同舟云学术

Determining the Genetic Structure of the Natural Population of Staphylococcus aureus : a Comparison of Multilocus Sequence Typing with Pulsed-Field Gel Electrophoresis, Randomly Amplified Polymorphic DNA Analysis, and Phage Typing

Published:2002-12 Issue:12 Volume:40 Page:4544-4546
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Grundmann Hajo¹,Hori Satoshi¹,Enright Mark C.²,Webster Carol¹,Tami Adriana¹,Feil Edward J.²,Pitt Tyrone³

Affiliation:

1. Division of Microbiology and Infectious Diseases, University Hospital Nottingham, Nottingham NG7 2UH

2. Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY

3. Laboratory of Hospital Infection, Division of Nosocomial Infection Prevention and Control, Central Public Health Laboratory, London NW9 5HT, United Kingdom

Abstract

ABSTRACT We used a sample of Staphylococcus aureus strains that are carried by humans and that are representative of the natural population of S. aureus strains in order to assess the value of multilocus sequence typing (MLST), pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing as epidemiological tools. Only MLST was able to define clonal complexes unambiguously. All DNA-based typing approaches achieved a high degree of agreement, implying phylogenetic concordance, but predicted epidemiological associations with variable accuracy.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.12.4544-4546.2002

Reference11 articles.

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