Highly sensitive biosensor based on IGZO thin-film transistors for detection of Parkinson's disease

Abstract

α-Synuclein (α-Syn) is a major biomarker of Parkinson's disease (PD). Concentration detection of α-Syn in cerebrospinal fluid and plasma of patients are used as a clue for early PD detection. Herein, we propose a thin-film transistor (TFT) biosensor based on an indium gallium zinc oxide (IGZO) thin film fabricated by the sol-gel method. The IGZO TFT biosensor is used for the early detection of PD after surface functionalization for α-Syn. The surface functionalization worked on the surface of the extended gate indium tin oxide sensing platform instead of the channel layer of TFT. Depending on concentrations of α-Syn in human cerebrospinal fluid and plasma, we diluted six different concentrations of α-Syn for detection, ranging from 1 pg ml−1 to 100 ng ml−1. The result shows that the biosensor exhibits high sensitivity and selectivity for α-Syn, a reliable sensing performance with a sensitivity of 189.9 mV dec−1, and a coefficient of determination R2 of 99.7% between 10 pg ml−1 and 100 ng ml−1.