Amino Acids as Gustatory Stimuli in the Rat

Abstract

Neural activity in intact chorda tympani nerve of rats was studied with an electronic summator. Neural activity increased when amino acid solutions 0.01 M or above passed over the tongue. Response magnitude, at concentrations close to solubility limits for the amino acids tested, was: DL-methionine < DL-tryptophan < DL-valine < DL-alanine < glycine < 0.1 M NaCl. Maximum response magnitudes to 1 M D-, and 1.2 M DL-alanine, and 1.5 M glycine developed in 1 to 3 minutes. Following such stimulation, a 63 per cent reduction in response to 0.1 M NaCl occurred 60 minutes after the first stimulation (medians). The depression was still present 20 hours later. Responses to glycine and alanine were not depressed. Amino acids vs. water preferences were investigated. With ascending concentration sequences, rats selected low concentration DL- and L-alanine and glycine; accepted D-, L-, and DL-tryptophan and low concentration DL-methionine; and rejected high concentration glycine, DL-alanine, and DL-methionine. Descending sequences showed depressed and delayed selection of glycine and DL-alanine, and DL-methionine and D- and L-tryptophan rejection. Both groups rejected DL-valine. It is concluded that glycine and alanine receptor effects differ from those of NaCl, but that all three compounds may affect a common receptor site. Prior exposure to amino acids may modify subsequent neural and/or behavioral responses.