Pharmacokinetic parameters of D-glucitol in laboratory animals in the context of assessing the functional state of the liver

Abstract

One of the most developing methods for assessing the state of the hepatobiliary system is the dynamic clearance method (assessment of clearance by studying pharmacokinetic parameters). Clearance is a measure of the rate at which a specific (usually most pharmacodynamically related to the system being studied) substance is eliminated from the body. This method allows not only to diagnose liver diseases, but also to determine their severity and the dynamics of changes in the process of further pharmacotherapy. The purpose of this study is to conduct an experimental study aimed at studying changes in the pharmacokinetics of exogenous sorbitol in the blood plasma of laboratory animals, which will establish starting points for further assessment of changes in its elimination properties in hepatobiliary pathologies. The level of Dglucitol was determined by the Corcoran and Page method with modification for 12 hours (step - 2 hours) in blood serum obtained by venipuncture of the tail vein, after administration of an experimental composition based on D-glucitol. Time intervals were selected based on the reported half-life of the drug. Based on the results of the study, we noted the following patterns in the biotransformation of D-glucitol: a linear increase in concentration up to 6 hours after administration (with a peak at 6 hours), a linear decrease in the period from 6 to 8 hours after administration, then a sharp decrease in concentration. Thus, the type of elimination of D-glucitol in clinically healthy animals can be assessed as “linear”. In the process of further research, it is planned to develop special “D-glucitol elimination curves”. These graphs will show the dynamics of exogenous D-glucitol removal over time in various hepatobiliary disorders.