Assessment of sorbitol clearance in induced acute liver failure

Abstract

The study of substance clearance plays an important role in the diagnosis of liver pathologies. Increased or decreased clearance of certain substances may indicate hepatobiliary disorders, including at the initial stages of the development of such pathologies, which allows us to evaluate the predictive potential of these assessment methods as high. The scientific literature provides detailed and statistically proven data on the use of substances such as galactose, amidopyrine, sorbitol and others in diagnostic protocols. Each substance is characterized by its own level of clearance, which allows one to obtain a comprehensive picture of the functioning of the hepatobiliary system. However, for most of the above exogenous substances, information in the scientific literature is either indicated in relation to humane medicine, or consists of pilot studies on a small number of animals, which requires additional testing of this kind to develop a statistically reliable array of data obtained. The purpose of this study is to conduct an experimental study aimed at studying changes in the pharmacokinetics of exogenous sorbitol in the blood plasma of laboratory animals with acute liver failure. The level of sorbitol was determined by the colorimetric Corcoran and Page method with modification (using potassium periodate and chromotropic acid, the method takes into account the calculated sorbitol indicator from the sum of polyols) using a UV-100 spectrophotometer (manufacturer – Shanghai Mapada Instruments Co., Ltd., China) for 12 hours (step – 2 hours) in blood plasma deproteinized using 0.4 M lithium perchlorate, obtained by venipuncture of the tail vein. Comparing pharmacokinetic curves with each other, it should be noted that in animals with acute liver failure, the graphical representation of sorbitol levels is similar to the inverse exponential, characterized by a sharp systematic decrease, while in clinically healthy animals the graphical representation of sorbitol levels is similar to the branch of a parabola, characterized by “smoothness” » reduction. The pharmacokinetic curves obtained in this study, which describe the levels of exogenous sorbitol in the blood plasma, can be used in the future as one of the methods for the early diagnosis of such pathologies.