Comparative Performance of Sanitizers in Managing Plant-to-Plant Transfer and Postharvest Infection of Calonectria pseudonaviculata and Pseudonectria foliicola on Boxwood

Abstract

Calonectria pseudonaviculata and Pseudonectria foliicola causing the infamous “boxwood blight” and “Volutella blight,” respectively, are a constant threat to the boxwood production and cut boxwood greenery market. Both pathogens cause significant economic loss to all parties (growers, retailer, and customers) in the horticultural chain. The objective of this study was to evaluate efficacy of disinfesting chemicals (quaternary ammonium compound [QAC], peroxy, acid, alcohol, chlorine, and cleaner) in preventing plant-to-plant transfer of C. pseudonaviculata and P. foliicola via cutting tools, as well as reduction of postharvest boxwood blight and Volutella blight disease severity in harvested boxwood greenery. First, an in vitro study was conducted to select products and doses that completely or near-completely inhibited conidial germination of C. pseudonaviculata and P. foliicola. The selected treatments were also tested for their ability to reduce plant-to-plant transfer of C. pseudonaviculata and P. foliicola and manage postharvest boxwood blight and Volutella blight in boxwood cuttings. For the plant-to-plant transfer study, Felco 19 shears were used as a tool for mechanical transfer of fungal conidia. The blades of Felco 19 shears were exposed to a conidial suspension of C. pseudonaviculata or P. foliicola by cutting a 1-cm-diameter cotton roll that had been dipped into a fungal suspension. Disease-free boxwood rooted cuttings (10-cm height) were pruned with the contaminated shears. The Felco 19 shears were equipped with a mounted miniature sprayer connected to a pressurized reservoir of treatment solution that automatically sprayed the blade and plant surface while cutting. The influence of accumulated sap on the shear blade was studied through 1- or 10-cut pruning variable on test plants and screened for the efficacy of treatments. Then, the boxwood rooted cuttings were transplanted and incubated in room conditions (21°C, 60% RH) with 12 h of fluorescent light; data evaluation on disease severity was done weekly for a month. Disease progress (area under disease progress curve [AUDPC]) was calculated. In another study, postharvest dip application treatments were used for the management of postharvest boxwood blight or Volutella blight on boxwood cuttings. The harvested boxwood cuttings were inoculated with a conidial suspension of C. pseudonaviculata or P. foliicola and then dipped into treatment solution 3 days afterward. The treated boxwood cuttings were kept in room conditions, and boxwood blight or Volutella blight disease severity as well as marketability (postharvest shelf life) was assessed every 2 days for 1 week. A significant difference between treatments was observed for reduction of boxwood blight or Volutella blight severity and AUDPC. The treatments [Octyl decyl dimethyl (ODD) + dioctyl dimethyl (DoD) + didecyl dimethyl (DdD) + dimethyl benzyl (DB)] ammonium chloride (AC) (Simple Green D Pro 5), 2-propanol + didecyl dimethyl ammonium chloride (DDAC) (0.12%; KleenGrow), and dimethyl benzyl ammonium chloride (DBAC) + dimethyl ethylbenzyl ammonium chloride (DEAC) (GreenShield) were the most effective in reducing the plant-to-plant transfer of boxwood blight and Volutella blight when pruned with contaminated Felco 19 shears. In addition to the three effective treatments above, acetic acid (2.5%; vinegar), 2-propanol + DDAC (0.06%), sodium hypochlorite (Clorox), and potassium peroxymonosulfate + NaCl (2%; Virkon) were effective in reducing postharvest boxwood blight, whereas DBAC + DBAC (Lysol all-purpose cleaner), ethanol (70% [ethyl alcohol]), and DDAC + DBAC (Simple Green D Pro 3 plus) were effective in reducing Volutella blight disease severity and AUDPC, and they also maintained better quality and longer postharvest shelf life of boxwood cuttings when applied as a dip treatment. The longer postharvest shelf life of boxwood cuttings noted may be attributed to reduced disease severity and AUDPC resulting in healthy boxwood cuttings.