Author:
Fu Longlong,An Qi,Zhang Kaishu,Liu Ying,Tong Yue,Xu Jianfeng,Zhou Fang,Wang Xiaowei,Guo Ying,Lu Wenhong,Liang Xiaowei,Gu Yiqun
Abstract
Abstract
Background
Human sperm cryopreservation is a simple and effective approach for male fertility preservation.
Methods
To identify potential proteomic changes in this process, data-independent acquisition (DIA), a technology with high quantitative accuracy and highly reproducible proteomics, was used to quantitatively characterize the proteomics of human sperm cryopreservation.
Results
A total of 174 significantly differential proteins were identified between fresh and cryoperservated sperm: 98 proteins decreased and 76 proteins increased in the cryopreservation group. Bioinformatic analysis revealed that metabolic pathways play an important role in cryopreservation, including: propanoate metabolism, glyoxylate and dicarboxylate metabolism, glycolysis/gluconeogenesis, and pyruvate metabolism. Four different proteins involved in glycolysis were identified by Western blotting: GPI, LDHB, ADH5, and PGAM1.
Conclusions
Our work will provide valuable information for future investigations and pathological studies involving sperm cryopreservation.
Funder
Fundamental Research Funds for the Central Universities
National Natural Science Foundation of China
Suzhou Science & Technology Planning Project
CAMS Innovation Fund for Medical Sciences
National “13th Five-Year” Plan for Science and Technology Support
Chinese Medical Association clinical medical research specific fund
Publisher
Springer Science and Business Media LLC
Subject
Urology,Reproductive Medicine,General Medicine
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