Abstract
Abstract
Background
Resveratrol, a phenolic compound, has various medicinal properties, including anticancer, anti-diabetic, antioxidant, anti-inflammatory, etc. Diabetes is a killing disease, especially for people who cannot maintain a healthy lifestyle. People with diabetes need additional information about a supplement that can prevent and treat diabetes. The present study aims to investigate the interaction of insulin with resveratrol using fluorescence spectroscopy, UV–Vis spectroscopy, CD spectropolarimeter, and molecular docking methods. As an outcome of this study, we expect to understand the contribution of resveratrol in insulin resistance.
Result
The fluorescence spectroscopy results showed that the peak intensity of insulin emission decreased with resveratrol. The interaction of insulin with resveratrol involved a combination of static and dynamic quenching effects. Temperature changes caused the binding constant (K) and the binding site (n) unstable. The interaction occurred through hydrogen bonding, van der Waal, and was hydrophobic. The results of UV–Vis spectroscopy showed that the addition of resveratrol caused a peak in a blueshift, and the absorbance was hyperchromic. Also, there was a reduction in electron transition and the extinction coefficient. The CD spectropolarimeter results showed that the addition of resveratrol affected the secondary structure of insulin. The amount of α-helix and β-sheet slightly change and increase in the secondary structure’s length. The molecular docking study showed that resveratrol interacts via hydrogen bonding with glycine and asparagine. van der Waal interactions occurred in asparagine, phenylalanine, and cysteine. The interaction of electrons occurred through the π orbitals of resveratrol with tyrosine A and B. The binding energy of molecules interaction happened spontaneously on a hydrophobic surface.
Conclusion
Profiling the interaction of insulin and resveratrol shows that resveratrol can stabilize insulin structure and prevent insulin resistance in diabetes.
Funder
This research was funded by the Department of Applied Chemistry Chaoyang University of Technology, Taiwan.
Publisher
Springer Science and Business Media LLC
Subject
Pharmaceutical Science,Agricultural and Biological Sciences (miscellaneous),Medicine (miscellaneous)
Cited by
3 articles.
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