Effect of arterial oxygenation on quadriceps fatigability during isolated muscle exercise

Abstract

The effect of various levels of oxygenation on quadriceps muscle fatigability during isolated muscle exercise was assessed in six male subjects. Twitch force (Qtw) was assessed using supramaximal magnetic femoral nerve stimulation. In experiment 1, maximal voluntary contraction (MVC) and Qtw of resting quadriceps muscle were measured in normoxia [inspired O2 fraction (FiO2) = 0.21, percent arterial O2 saturation (Sp[Formula: see text]) = 98.4%, estimated arterial O2 content (CaO2) = 20.8 ml/dl], acute hypoxia (FiO2 = 0.11, Sp[Formula: see text] = 74.6%, CaO2 = 15.7 ml/dl), and acute hyperoxia (FiO2 = 1.0, Sp[Formula: see text] = 100%, CaO2 = 22.6 ml/dl). No significant differences were found for MVC and Qtw among the three FiO2 levels. In experiment 2, the subjects performed three sets of nine, intermittent, isometric, unilateral, submaximal quadriceps contractions (62% MVC followed by 1 MVC in each set) while breathing each FiO2. Qtw was assessed before and after exercise, and myoelectrical activity of the vastus lateralis was obtained during exercise. The percent reduction of twitch force (potentiated Qtw) in hypoxia (−27.0%) was significantly ( P < 0.05) greater than in normoxia (−21.4%) and hyperoxia (−19.9%), as were the changes in intratwitch measures of contractile properties. The increase in integrated electromyogram over the course of the nine contractions in hypoxia (15.4%) was higher ( P < 0.05) than in normoxia (7.2%) or hyperoxia (6.7%). These results demonstrate that quadriceps muscle fatigability during isolated muscle exercise is exacerbated in acute hypoxia, and these effects are independent of the relative exercise intensity.