Microvascular reperfusion injury: rapid expansion of anatomic no reflow during reperfusion in the rabbit

Author:

Reffelmann Thorsten1,Kloner Robert A.1

Affiliation:

1. The Heart Institute, Good Samaritan Hospital, University of Southern California, Los Angeles, California 90017-2395

Abstract

The aim was to define the degree and time course of reperfusion-related expansion of no reflow. In five groups of anesthetized, open-chest rabbits (30-min coronary occlusion and different durations of reperfusion), anatomic no reflow was determined by injection of thioflavin S at the end of reperfusion and compared with regional myocardial blood flow (RMBF; radioactive microspheres) and infarct size (triphenyltetrazolium). The area of no reflow progressively increased from 12.2 ± 4.2% of the risk area after 2 min of reperfusion to 30.8 ± 3.1% after 2 h and 34.9 ± 3.3% after 8 h and significantly correlated with infarct size after 1 h of reperfusion ( r = 0.88–0.97). This rapid expansion of no reflow predominantly occurred during the first 2 h, finally encompassing ∼80% of the infarct size, and was accompanied by a decrease of RMBF within the risk area, being hyperemic after 2 min of reperfusion (3.78 ± 0.75 ml · min−1 · g−1) and plateauing at a level of ∼0.9 ml · min−1 · g−1 by 2 and 8 h of reperfusion (preischemic RMBF: 2.06 ± 0.01 ml · min−1 · g−1). The development of macroscopic hemorrhage lagged behind no reflow, was closely correlated with it, and may be the consequence of microvascular damage.

Publisher

American Physiological Society

Subject

Physiology (medical),Cardiology and Cardiovascular Medicine,Physiology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3