Dietary Effect of Grape Seed Proanthocyanidin Extract on Growth Performance, Serum Biochemical Parameters, Skin Mucosal Immune Response, and Antioxidant Capacity in Goldfish (Carassius auratus)

Abstract

Abstract A trial was conducted to evaluate the effect of dietary grape seed proanthocyanidin extract (GSPE) on goldfish, Carassius auratus. In this regard, GSPE was added to a basal diet at four levels including 0, 200, 400, and 600 mg/kg to produce four experimental diets including control, GSPE200, GSPE400, and GSPE600. Three hundred and sixty goldfish (3.75 ± 0.1 g) were stocked in twelve 100 L rectangular tanks (30 fish per tank) and fed with the experimental diets three times a day for nine weeks. During the experimental trial, water temperature was 26.7–28.5°C. The weight gain and specific growth rate in the fish fed with GSPE supplemented diets were higher than the control, meanwhile feed conversion ratio value in these groups decreased compared to the control. Fish fed GSPE-supplemented diets had lower fillet lipid (10–19%), but higher protein levels (7–15%) compared to the control. The levels of serum triglyceride, alanine aminotransferase and aspartate aminotransferase in the fish fed GSPE-supplemented diets were decreased compared to the control group. The highest and lowest levels of serum glucose, and ALP were in the fish fed with control and GSPE600 diets, respectively. The skin mucosal lysozyme activity (24–38%) and protein level (70–96%) were higher in fish fed GSPE-supplemented diets than the control. The highest, and lowest liver antioxidant enzymes including superoxide dismutase, catalase, and glutathione peroxidase were observed in GSPE 600 and control groups, respectively. The findings of the present study indicated that supplementing 400 mg/kg GSPE in diet can improve growth and health condition in goldfish.