A single point mutation converts a proton-pumping rhodopsin into a red-shifted, turn-on fluorescent sensor for chloride-Reference-Cited by-同舟云学术

A single point mutation converts a proton-pumping rhodopsin into a red-shifted, turn-on fluorescent sensor for chloride

Published:2021 Issue:15 Volume:12 Page:5655-5663
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Tutol Jasmine N.¹²³⁴^ORCID,Lee Jessica¹²³⁴⁵,Chi Hsichuan¹²³⁴⁵,Faizuddin Farah N.¹²³⁴⁵,Abeyrathna Sameera S.¹²³⁴,Zhou Qin⁵²³⁴,Morcos Faruck⁵²³⁴⁶,Meloni Gabriele¹²³⁴^ORCID,Dodani Sheel C.¹²³⁴^ORCID

Affiliation:

1. Department of Chemistry and Biochemistry

2. The University of Texas at Dallas

3. Richardson

4. USA

5. Department of Biological Sciences

6. Department of Bioengineering

Abstract

By utilizing laboratory-guided evolution, we have converted the fluorescent proton-pumping rhodopsin GR from Gloeobacter violaceus into GR1, a red-shifted, turn-on fluorescent sensor for chloride.

Funder

National Institute of General Medical Sciences

National Science Foundation

Welch Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/SC/D0SC06061E

Reference101 articles.

1. U. Lindh , in Essentials of Medical Geology , ed. O. Selinus , Springer , Dordrecht , 2nd edn, 2013 , ch. 7, pp. 129–177