Addition of a polyhistidine tag alters the regioselectivity of carbonyl reductase S1 from Candida magnoliae-Reference-Cited by-同舟云学术

Addition of a polyhistidine tag alters the regioselectivity of carbonyl reductase S1 from Candida magnoliae

Published:2017 Issue:48 Volume:15 Page:10256-10264
ISSN:1477-0520
Container-title:Organic & Biomolecular Chemistry
language:en
Short-container-title:Org. Biomol. Chem.

Author:

Haas Julian¹²³⁴,Häckh Matthias¹²³⁴,Justus Viktor¹²³⁴,Müller Michael¹²³⁴,Lüdeke Steffen¹²³⁴^ORCID

Affiliation:

1. Institute of Pharmaceutical Sciences

2. University of Freiburg

3. 79104 Freiburg

4. Germany

Abstract

A recombinant carbonyl reductase shows different regioselectivity with a C-terminal His-tag compared to the N-tagged enzyme toward the same triketide substrate. Highly selective synthesis of reference triketides allowed solving this conundrum.

Funder

Deutsche Forschungsgemeinschaft

Publisher

Royal Society of Chemistry (RSC)

Subject

Organic Chemistry,Physical and Theoretical Chemistry,Biochemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2017/OB/C7OB02666H

Reference46 articles.

1. C. Hertweck, Angew. Chem. Int. Ed. , 2009, 48 , 4688

2. P. F. Leadlay, Chem. Biol. , 2006, 13 , 287

3. M. Häckh, M. Müller and S. Lüdeke, Chem. – Eur. J. , 2013, 19 , 8922

4. A fungal ketoreductase domain that displays substrate-dependent stereospecificity

5. Enzymatic Reductions for the Regio- and Stereoselective Synthesis of Hydroxy-keto Esters and Dihydroxy Esters

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