A single-phase flow microfluidic cell sorter for multiparameter screening to assist the directed evolution of Ca2+ sensors-Reference-Cited by-同舟云学术

A single-phase flow microfluidic cell sorter for multiparameter screening to assist the directed evolution of Ca2+ sensors

Published:2019 Issue:22 Volume:19 Page:3880-3887
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Zhao Yufeng¹²³⁴^ORCID,Zhang Wei¹²³⁴,Zhao Yongxin¹²³⁴,Campbell Robert E.¹²³⁴¹^ORCID,Harrison D. Jed¹²³⁴

Affiliation:

1. Department of Chemistry

2. University of Alberta

3. Edmonton

4. Canada

Abstract

We introduce a single-phase flow microfluidic cell sorter with a two-point detection system capable of two-parameter screening to assist with directed evolution of a fluorescent protein based Ca²⁺ sensor expressed in bacterial cells.

Funder

Natural Sciences and Engineering Research Council of Canada

Canadian Institutes of Health Research

Foundation for the National Institutes of Health

Alberta Innovates - Technology Futures

Fondation Brain Canada

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2019/LC/C9LC00779B

Reference28 articles.

1. Picoliter Cell Lysate Assays in Microfluidic Droplet Compartments for Directed Enzyme Evolution

2. Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

3. Droplet microfluidic technology for single-cell high-throughput screening

4. A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution

5. Quantitative and sensitive detection of rare mutations using droplet-based microfluidics

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