An automated screening platform for improving the responsiveness of genetically encoded Ca<sup>2+</sup> biosensors in mammalian cells-Reference-Cited by-同舟云学术

An automated screening platform for improving the responsiveness of genetically encoded Ca²⁺ biosensors in mammalian cells

Published:2024 Issue:9 Volume:3 Page:1494-1504
ISSN:2635-0998
Container-title:Sensors & Diagnostics
language:en
Short-container-title:Sens. Diagn.

Author:

Zhao Yufeng¹²³^ORCID,Shen Yi¹^ORCID,Veres Teodor²⁴,Campbell Robert E.¹⁵⁶

Affiliation:

1. Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada

2. Centre for Research and Applications in Fluidic Technologies, National Research Council of Canada, Toronto, Ontario M5S 3G8, Canada

3. Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Ontario M5S 3M2, Canada

4. Medical Devices Research Center, Life Sciences Division, National Research Council of Canada, Boucherville, QC J4B 6Y4, Canada

5. Department of Chemistry, Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan

6. CERVO Brain Research Center and Department of Biochemistry, Microbiology, and Bioinformatics, Université Laval, Québec, Québec G1V 0A6, Canada

Abstract

We developed a novel automated platform to screen the responsiveness of fluorescent protein (FP)-based biosensors to chemical stimulation in mammalian cells and discovered improved red fluorescent Ca2+ biosensor variants using this platform.

Funder

Natural Sciences and Engineering Research Council of Canada

Japan Society for the Promotion of Science

Canadian Institutes of Health Research

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/SD/D4SD00138A

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