Author:
Sierra Raymond G.,Laksmono Hartawan,Kern Jan,Tran Rosalie,Hattne Johan,Alonso-Mori Roberto,Lassalle-Kaiser Benedikt,Glöckner Carina,Hellmich Julia,Schafer Donald W.,Echols Nathaniel,Gildea Richard J.,Grosse-Kunstleve Ralf W.,Sellberg Jonas,McQueen Trevor A.,Fry Alan R.,Messerschmidt Marc M.,Miahnahri Alan,Seibert M. Marvin,Hampton Christina Y.,Starodub Dmitri,Loh N. Duane,Sokaras Dimosthenis,Weng Tsu-Chien,Zwart Petrus H.,Glatzel Pieter,Milathianaki Despina,White William E.,Adams Paul D.,Williams Garth J.,Boutet Sébastien,Zouni Athina,Messinger Johannes,Sauter Nicholas K.,Bergmann Uwe,Yano Junko,Yachandra Vittal K.,Bogan Michael J.
Abstract
An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14–3.1 µl min−1to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min−1and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.
Publisher
International Union of Crystallography (IUCr)
Subject
General Medicine,Structural Biology
Cited by
169 articles.
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