High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography-Reference-Cited by-同舟云学术

High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography

Published:2012-07-20 Issue:6092 Volume:337 Page:362-364
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Boutet Sébastien¹,Lomb Lukas²³,Williams Garth J.¹,Barends Thomas R. M.²³,Aquila Andrew⁴,Doak R. Bruce⁵,Weierstall Uwe⁵,DePonte Daniel P.⁴,Steinbrener Jan²³,Shoeman Robert L.²³,Messerschmidt Marc¹,Barty Anton⁴,White Thomas A.⁴,Kassemeyer Stephan²³,Kirian Richard A.⁵,Seibert M. Marvin¹,Montanez Paul A.¹,Kenney Chris⁶,Herbst Ryan⁶,Hart Philip⁶,Pines Jack⁶,Haller Gunther⁶,Gruner Sol M.⁷⁸,Philipp Hugh T.⁷,Tate Mark W.⁷,Hromalik Marianne⁹,Koerner Lucas J.¹⁰,van Bakel Niels¹¹,Morse John¹²,Ghonsalves Wilfred¹,Arnlund David¹³,Bogan Michael J.¹⁴,Caleman Carl⁴,Fromme Raimund¹⁵,Hampton Christina Y.¹⁴,Hunter Mark S.¹⁵,Johansson Linda C.¹³,Katona Gergely¹³,Kupitz Christopher¹⁵,Liang Mengning⁴,Martin Andrew V.⁴,Nass Karol¹⁶,Redecke Lars¹⁷¹⁸,Stellato Francesco⁴,Timneanu Nicusor¹⁹,Wang Dingjie⁵,Zatsepin Nadia A.⁵,Schafer Donald¹,Defever James¹,Neutze Richard¹³,Fromme Petra¹⁵,Spence John C. H.⁵,Chapman Henry N.⁴¹⁶,Schlichting Ilme²³

Affiliation:

1. Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA.

2. Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany.

3. Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.

4. Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron (DESY), Notkestrasse 85, 22607 Hamburg, Germany.

5. Department of Physics, Arizona State University, Tempe, AZ 85287, USA.

6. Particle Physics and Astrophysics, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA.

7. Department of Physics, Laboratory of Atomic and Solid State Physics, Cornell University, Ithaca, NY 14853, USA.

8. Wilson Laboratory, Cornell High Energy Synchrotron Source (CHESS), Cornell University, Ithaca, NY 14853, USA.

9. Electrical and Computer Engineering, State University of New York (SUNY) Oswego, Oswego, NY 13126, USA.

10. The Johns Hopkins University Applied Physics Laboratory, 11100 Johns Hopkins Road, Laurel, MD 20723, USA.

11. Nikhef, National Institute for Subatomic Physics, Science Park 105, 1098 XG Amsterdam, Netherlands.

12. European Synchrotron Radiation Facility, 38043 Grenoble Cedex, France.

13. Department of Chemistry and Molecular Biology, University of Gothenburg, SE-405 30 Gothenburg, Sweden.

14. PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA.

15. Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287–1604, USA.

16. University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.

17. Joint Laboratory for Structural Biology of Infection and Inflammation, Institute of Biochemistry and Molecular Biology, University of Hamburg, and Institute of Biochemistry, University of Lübeck, at DESY, Hamburg, Germany.

18. German Centre for Infection Research, University of Lübeck, 23538 Lübeck, Germany.

19. Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.

Abstract

Size Matters Less X-ray crystallography is a central research tool for uncovering the structures of proteins and other macromolecules. However, its applicability typically requires growth of large crystals, in part because a sufficient number of molecules must be present in the lattice for the sample to withstand x-ray—induced damage. Boutet et al. (p. 362 , published online 31 May) now demonstrate that the intense x-ray pulses emitted by a free-electron laser source can yield data in few enough exposures to uncover the high-resolution structure of microcrystals.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference25 articles.

1. The minimum crystal size needed for a complete diffraction data set

2. Potential for biomolecular imaging with femtosecond X-ray pulses

3. Self-terminating diffraction gates femtosecond X-ray nanocrystallography measurements

4. Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser

5. Gas dynamic virtual nozzle for generation of microscopic droplet streams

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