A pre-screening FISH-based method to detect CRISPR/Cas9 off-targets in mouse embryonic stem cells
Author:
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/srep12327.pdf
Reference26 articles.
1. Jinek, M. et al. RNA-programmed genome editing in human cells. eLife 2, e00471, 10.7554/eLife.00471 (2013).
2. Mali, P. et al. RNA-guided human genome engineering via Cas9. Science 339, 823–826 (2013).
3. Harrison, M. M., Jenkins, B. V., O’Connor-Giles, K. M. & Wildonger, J. A CRISPR view of development. Gene Dev 28, 1859–1872 (2014).
4. Lin, Y. et al. CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences. Nucleic Acids Res 42, 7473–7485 (2014).
5. Kuscu, C., Arslan, S., Singh, R., Thorpe, J. & Adli, M. Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease. Nat Biotechnol 32, 677–683 (2014).
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