Author:
Harrison Melissa M.,Jenkins Brian V.,O’Connor-Giles Kate M.,Wildonger Jill
Abstract
The CRISPR (clustered regularly interspaced short palindromic repeat)–Cas9 (CRISPR-associated nuclease 9) system is poised to transform developmental biology by providing a simple, efficient method to precisely manipulate the genome of virtually any developing organism. This RNA-guided nuclease (RGN)-based approach already has been effectively used to induce targeted mutations in multiple genes simultaneously, create conditional alleles, and generate endogenously tagged proteins. Illustrating the adaptability of RGNs, the genomes of >20 different plant and animal species as well as multiple cell lines and primary cells have been successfully modified. Here we review the current and potential uses of RGNs to investigate genome function during development.
Funder
National Institute of Neurological Disorders and Stroke, National Institutes of Health
Publisher
Cold Spring Harbor Laboratory
Subject
Developmental Biology,Genetics
Cited by
184 articles.
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