Antibody-coupled siRNA as an efficient method for in vivo mRNA knockdown-Reference-Cited by-同舟云学术

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Antibody-coupled siRNA as an efficient method for in vivo mRNA knockdown

Published:2015-12-03 Issue:1 Volume:11 Page:22-36
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Bäumer Nicole,Appel Neele,Terheyden Lisa,Buchholz Frank,Rossig Claudia,Müller-Tidow Carsten,Berdel Wolfgang E,Bäumer Sebastian

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2015.137.pdf

Reference27 articles.

1. Bäumer, S. et al. Antibody-mediated delivery of anti-KRAS-siRNA in vivo overcomes therapy resistance in colon cancer. Clin. Cancer Res. 21, 1383–1394 (2015).

2. Choi, Y.S. et al. The systemic delivery of siRNAs by a cell penetrating peptide, low-molecular-weight protamine. Biomaterials 31, 1429–1443 (2009).

3. El-Andaloussi, S. et al. Exosome-mediated delivery of siRNA in vitro and in vivo. Nat. Protoc. 7, 2112–2126 (2012).

4. Liu, B. Exploring cell type-specific internalizing antibodies for targeted delivery of siRNA. Brief. Funct. Genomics Proteomics 6, 112–119 (2007).

5. Hsu, C.Y. & Uludag, H. A simple and rapid nonviral approach to efficiently transfect primary tissue-derived cells using polyethylenimine. Nat. Protoc. 7, 935–945 (2012).

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